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-groupsize: 5 is a good number. Increasing this number will make better references, but they may also make them less accurate, as you would be averaging a larger number of profiles. If you decide to increase this number by a factor, then I would also increase n by the same factor, so in the end the total number of reference is the same. (see also thoughts from Tim Maxwell below).

-islandsplitmethod: (default value: 'scipylabel') Several image processing algorithms have been created to separate bunches that appear on the same image. Each algorithm is advantageous in certain types of conditions and thus it might be worth trying all of them and see what achieves the best results.  The defaults parameter is 'scipylabel'. If one does not set islandsplitmethod to anything, the parameter will be set to 'scipylabel'. If one would like to change from the default , one can use GLOC.islandsplitmethod = 'contourLabel'.  'scipylabel' calls the scipy label function to label contiguous regions.  See here for some algorithm details.

• 'scipylabel' calls the scipy label function to label contiguous regions.  this is fast, but requires a region of no signal (after de-noising) between the regions.
• 'autothreshold' tries to separate the islands by automatically finding thresholds that separate them.  this is the recommended mode for multi-bunch operation.
• 'contourlabel' tries to adjust a threshold until two large groups are found, grouping together pixels using an opencv contour method.  there are two parameters ('ratio1/ratio2') that are settable for the contour method

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• that determine

-roexpand and roiwaistthres: Call the method 'ProcessedXTCAVImage'. If the image does not look clipped, the parameters are fine.

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